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Recombinant Trypsin Pharmacopo

  • Date Listed:2021-03-04 14:22:01
    Country:China>Zhejiang>Hangzhou
    Xuxian Industrial Park,Yiqiao Town,Xiaoshan District,Hangzhou City,Zhejiang Province,China
    Company:Hangzhou Putai Biotechnology Co.,Ltd
    Mobile:+8615558122137
    Tell:+86-0571-82359562

    Recombinant Trypsin Pharmacopoeia grade(Freeze-dried powder) CAS: 9002-07-7 Recombinant Trypsin Specifications:10mg;100mg;1g;packing specifications can be customized according to customer needs I.Product introduction Recombinant Trypsin: CAS: 9002-07-7; EC: 3.4.21.4 Recombinant Trypsin is a serine protease, which can specifically cleave the C-terminal peptide end of lysine and arginine. Trypsin is widely used in various biotechnology processes. For example: proteolysis to produce polypeptides; cell separation of various tissues; proteolysis and sequencing; recombinant insulin production and so on. II. Product characteristics Source: Pichia pastoris Character: white or similar white freeze-dried powder Purity :(RP-HPLC): β-trypsin≥70%,α-trypsin ≤20% Specific activity: ≥ 3800 USP units / mg Pro Host protein residue: ≤0.01% Host cell DNA residue: ≤ 10 ng/mg Specificity: cleavage of the carboxy terminal peptide bond between arginine and lysine. III. Product use Production of recombinant insulin and analogs; cell culture and fermentation; enzymatic hydrolysis of proteins; cell separation of various tissues. Iv. Recommend Usage It is recommended to use 1mM HCl solution to dissolve, The ration to aimed protein is 1:50 to 1:1000, optimal ph 7.0-9.0, optimal temperature 25-35℃. Note: The activity is inhibited by serine protease inhibitors such as PMSF, metal ion chelating agents such as EDTA, etc.; high concentration of inorganic salts will have certain effects on enzyme activity. V. Storage stability The lyophilized powder can be stored for more than 2 years at temperatures below –15 °C. Vi. Product advantages Animal origin free: Recombinant production, no exogenous virus contamination. No animal-derived material is used during production. Stable quality: stable and continuous batch production can be guaranteed; There is no difference between product batches and the quality is stable. Lyophilized powder: easy to store and tran....

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